Journal: Adipocyte
Article Title: Optimization of in vitro cultivation strategies for human adipocyte derived stem cells
doi: 10.4161/21623945.2014.987580
Figure Lengend Snippet: (A) Cells cultured in BM 2 (right) show a higher proliferation rate during a 24-h cultivation period than cells cultivated in BM 1 (left). Each value represents the mean ± SD of triplicate values from 4 different ADSC-Preparations. Absorbance values obtained with medium alone (<0.02) were taken as the control and subsequently subtracted from all measurements. (B) Microscopic examination of ADSC cultured in 2 different culture media. 4 representative phase-contrast images are shown. left: ADSC were seeded at a density of 10.000 in DMEM/F12 medium (BM1) in 10 cm2 round tissue culture dishes and photographed 24 and 48 hours after seeding. Right: ADSC were seeded at a density of 10.000 in ECGM medium (BM2) in 10 cm2 round tissue culture dishes and photographed 24 and 48 hours after seeding.
Article Snippet: The isolated preadipocytes were cultured both in BM 1 as described above, and in a specialized endothelial cell growth medium (ECGM), BM2, which was supplied by Promocell and whose exact content will remain undisclosed because of the patent rights of the manufacturer.
Techniques: Cell Culture